Cardiovascular and Metabolic Effects of ANGPTL3 Antisense Oligonucleotides

Neuroendocrine and behavioral effects of antisense oligonucleotides.

In addition to traditional morphological and functional strategies, including physiological and pharmacological tools, innovative approaches are necessary to investigate molecular, cellular and neuroendocrine correlates of behavior. Neuroendocrine as well as behavioral regulation, particularly in mammals, is a priori complex at multiple levels, justifying the use of various complementary techni...

Antisense Oligonucleotides: Strategies and Applications

Designing Antisense Oligonucleotides

Antisense Oligonucleotides Targeting Protein

Designing Antisense Oligonucleotides

Antisense oligonucleotides have been used for a number of years to modify the expression of specific genes both in vivo and in vitro (Scanlon et al., 1995). The most potent mode of antisense activity is through RNase H–mediated degradation of RNA (Figure 1). The RNase H– endonuclease specifically cleaves RNA only when it is hybridized as a heteroduplex with DNA. In general, oligonucleotides tha...